購物車
幫助
021-54845833/15800441009
|
Background: |
Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192). |
|
Applications: |
WB, IHC |
|
Name of antibody: |
FZR1 |
|
Immunogen: |
Fusion protein of human FZR1 |
|
Full name: |
fizzy and cell division cycle 20 related 1 |
|
Synonyms: |
FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C |
|
SwissProt: |
Q9UM11 |
|
IHC positive control: |
Human breast cancer and Human esophagus cancer |
|
IHC Recommend dilution: |
25-100 |
|
WB Predicted band size: |
55 kDa |
|
WB Positive control: |
Human placenta tissue |
|
WB Recommended dilution: |
500-2000 |
